大发快三高手计划

    

方显杨 博士

研究员


2002年/武汉大学/学士

2008年/中国科大发快三高手计划生物物理研究所/博士

2009-2015年/美国国立卫生研究院国立癌症研究所/博士后

2015年-至今/清华大学生命科学大发快三高手计划/研究员

 

●  研究兴趣、领域:


本课题组长期致力于与人类疾病或重大生命过程密切相关的非编码RNA(包括黄病毒,艾滋病毒与冠状病毒来源的RNA,lncRNA)的整合结构生物学研究。一方面整合运用小角X射线/中子散射技术、电子顺磁共振/核磁共振技术、X射线晶体学、单分子荧光共振能量转移、单分子生物纳米孔技术以及计算模拟,研究长链RNA的三维空间结构、动态特性与功能的关系;另一方面致力于发展RNA的体外与活细胞内位点特异性与区段选择性标记技术,探索活细胞内RNA的结构研究策略与实验方案。

●  代表性论文:


1. Xiaolin Niu#, Qiuhan Liu#, Zhonghe Xu#, Zhifeng Chen, Linghui Xu, Lilei Xu, Jinghong Li*, Xianyang Fang*. Molecular mechanisms underlying the mechanical anisotropy of flaviviral exoribonuclease-resistant RNAs (xrRNAs). Nature Communications 2020, 11(1): 5496

2. Yan Wang, Venkatesan Kathiresan, Yaoyi Chen, Yanping Hu, Wei Jiang, Guangcan Bai, Guoquan Liu, Peter Z. Qin*, Xianyang Fang.* Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions. Chemcial Science 2020, 11: 9655-9664.

3. Yan Wang, Yaoyi Chen, Yanping Hu, Xianyang Fang. Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription. Proceedings of the Naitonal Academy of Sciences 2020, 117(37): 22823-22832.

4. Yupeng Zhang#, Yikan Zhang#, Zhong-Yu Liu#,Meng-Li Cheng#, Junfeng Ma, Yan Wang, Cheng-Feng Qin*, Xianyang Fang*. Long non-coding subgenomic flavivirus RNAs have extended 3D structures and are flexible in solution. EMBO Reports 2019, 20(11): e47016.

5. Xianyang Fang, Jason R. Stagno,Yuba Bh.andari,Xiaobing Zuo,Yun-Xing Wang. Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Current Opinion in Structural Biology 2015, 30: 147-160.

6. Xianyang Fang# , Jinbu Wang#, Ina P. O’Carroll#, Michelle Mitchell, Xiaobing Zuo, Yi Wang, Ping Yu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, J?rgen Kjems, Charles D. Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, Alan Rein*,Yun-Xing Wang*. An unusual topological structure of the HIV-1 rev response element, Cell 2013, 155(3): 594-605.

( #co-first author,*corresponding author)

●  联系方式:


电话:86-010-62771071

邮箱:fangxy@mail.tsinghua.edu.cn

通讯地址:清华大学大发快三高手计划科学楼B1004